Regulatory

Part:BBa_K588001:Design

Designed by: Kevin Lu   Group: iGEM11_Rutgers   (2011-09-26)

ptrp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains SpeI sites at positions critical for function. We modified the SpeI site in the biobrick suffix to an NheI site as done by UNAM 2010

We had this part synthesized and kinased such that the strands could be annealed and dropped into the linearized pSB1C3.

Strand sequences
5'- AATTCGCGGCCGCTTCTAGAGGCTGTTGACAATTAATCATCGAACTAGTTAACTAGTACGCAAGTTCACGTGCTAGCAGCGGCCGCTGCA -3' (90 bp)
3'- GCGCCGGCGAAGATCTCCGACAACTGTTAATTAGTAGCTTGATCAATTGATCATGCGTTCAAGTGCACGATCGTCGCCGGCG -5' (82 bp)

Source

Synthesized by IDT

References